First take a deep breath and know that you’re doing great! We know that many of us put a lot of pressure on ourselves to make sure we are doing everything we can to improve our chances on our journey to conceive.
Air bubbles are more of a nuisance than anything. As long as you've pushed any big air bubbles out you’re ok. The reason we bring it up in our instructions is to make everyone aware that there are a few things they can do that can help eliminate some of the bubbles, but it's ultimately impossible to remove all of them (as you may know!).
In our own case there were a few tiny air bubbles and we also expelled a tiny amount of sperm in the effort to push it to the tip of the barrel. Ultimately, most of the sample made the journey and that's what really matters. Remember it only takes one. And speaking frankly, the day we tried there was not a lot of his stuff to work with and we were both quite surprised to see a positive!
Hope this helps and, try not to overthink it, as long as most of the sample is successfully transferred. We are here to help and support and if you have any other questions please do feel free to email us at mosie@mosiebaby.com. Our best to you!